abstract：:Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...

journal_title：Molecular immunology

authors： Gibson DM

更新日期：1984-05-01 00:00:00

abstract：:Hyperimmune absorbed rabbit antisera which were reactive with epitopes specific for individual variants of human placental alkaline phosphatase were tested for their reactivity with primate placental alkaline phosphatases. Using the three epitope-specific reactivities defined previously, we found that: epitope I is pr...

journal_title：Molecular immunology

authors： Doellgast GJ,Wei SC

更新日期：1984-03-01 00:00:00

abstract：:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...

journal_title：Molecular immunology

authors： Wilkinson JM,Wetterskog DL,Sogn JA,Kindt TJ

更新日期：1984-01-01 00:00:00

abstract：:Anaerobic reduction of purified rabbit IgG antibody (Ab) with 1.5 moles of dithiothreitol per mole of Ab at pH 8.0, followed by alkylation, cleaves 39% of the inter-heavy-chain (H-H) disulfide (SS) bonds. This treatment has the following effects on the ability of the Ab to activate the classical pathway of complement....

journal_title：Molecular immunology

authors： Johnson BA,Hoffmann LG

更新日期：1984-01-01 00:00:00

abstract：:Effective immunization with short polypeptide antigens has typically only been possible when the peptide is conjugated to a large carrier substance, usually a protein. Such immunizations suffer from difficulties in producing conjugates of reliable composition, and from unwanted anti-carrier immune responses. When a ch...

journal_title：Molecular immunology

authors： Hopp TP

更新日期：1984-01-01 00:00:00

abstract：:Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...

journal_title：Molecular immunology

authors： Clark SK Jr,Conroy JM,Harris PJ

更新日期：1983-12-01 00:00:00

abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...

journal_title：Molecular immunology

authors： Boackle RJ,Caughman GB,Vesely J,Medgyesi G,Fudenberg HH

更新日期：1983-11-01 00:00:00

abstract：:Human gastrointestinal glycoproteins (mucins), isolated by pepsin digestion from foetal stomachs and meconia, and from paired tumour and non-neoplastic mucosal samples of patients with gastric and colorectal carcinomas, were tested for precipitating reactions with peanut lectin (PNL) and four anti-carbohydrate antibod...

journal_title：Molecular immunology

authors： Picard JK,Feizi T

更新日期：1983-11-01 00:00:00

abstract：:Glycophorin, one of the major glycoproteins of erythrocytes (E), was extracted from human E (glycophorin-Hu) and guinea pig E (glycophorin-GP) and adsorbed to rabbit-E. The adsorption of glycophorin-Hu and glycophorin-GP to rabbit-E made the E resistant to hemolysis by human serum and guinea pig serum, respectively, v...

journal_title：Molecular immunology

authors： Okada H,Tanaka H

更新日期：1983-11-01 00:00:00

abstract：:Serum levels of IgA1 and IgA2 were measured by solid phase radioimmunoassay in samples from 110 children between 3 months and 10 years of age. Both IgA1 and IgA2 were detectable in all samples, and both IgA1 and IgA2 increased with increasing age. The percent of total serum IgA that was IgA2 did not change with age an...

journal_title：Molecular immunology

authors： Conley ME,Arbeter A,Douglas SD

更新日期：1983-09-01 00:00:00

abstract：:Symmetry in antibody plus complement mediated killing between a T15 idiotype-bearing monoclonal antibody (HPC-M2) and an anti-T15 monoclonal antibody (B36-82) is demonstrated. The HPC-M2 antibody is also shown to be multispecific in its effector function, since it lyses both phosphorylcholine coated erythrocytes, and ...

journal_title：Molecular immunology

authors： Cooper-Willis A,Hoffmann GW

更新日期：1983-08-01 00:00:00

abstract：:Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of...

journal_title：Molecular immunology

authors： Kulics J,Rajnavölgyi E,Füst G,Gergely J

更新日期：1983-08-01 00:00:00

abstract：:This report demonstrates that a marker of human embryonic endoderm and embryonal carcinoma cells recognized by a hybridoma antibody FC 10.2, involves Type 1 blood group chains with the sequence Gal beta 1 leads to 3G1cNAc beta 1 leads to 3Gal beta 1 leads to 4G1c. This conclusion has been reached from antigenic analys...

journal_title：Molecular immunology

authors： Gooi HC,Williams LK,Uemura K,Hounsell EF,McIlhinney RA,Feizi T

更新日期：1983-06-01 00:00:00

abstract：:Previous investigation have demonstrated the presence of the Rho(D) antigen in Rh negative erythrocytes. The intact Rh negative cell, however, does not bind anti-D IgG. Presently we have shown that an anti-D binding antigen resides on the cytoplasmic surface of Rh negative erythrocyte membranes. Unsealed Rh negative m...

journal_title：Molecular immunology

authors： Evans JP,Brown PJ,Sinor LT,Tilzer LL,Beck M,Plapp FV

更新日期：1983-05-01 00:00:00

abstract：:A computerized method for predicting the locations of protein antigenic determinants is presented, which requires only the amino acid sequence of a protein, and no other information. This procedure has been used to predict the major antigenic determinant of the hepatitis B surface antigen, as well as antigenic sites o...

journal_title：Molecular immunology

authors： Hopp TP,Woods KR

更新日期：1983-04-01 00:00:00

abstract：:The discovery of a human myeloma protein comprising a kappa L-chain with an increased mol. wt of 30,000) (Bouvet et. al., 1980) prompted investigations on the incidence of such heavier L-chains among other human myeloma proteins. In 105 samples examined, 34 were found to have L-chains heavier than normal (23,000-24,00...

journal_title：Molecular immunology

authors： Bouvet JP,Pillot J,Liacopoulos P

更新日期：1983-04-01 00:00:00

abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...

journal_title：Molecular immunology

authors： Pink JR,Hoessli D,Tartakoff A,Hooghe R

更新日期：1983-04-01 00:00:00

abstract：:Subclasses of IgG were separated from pools of mouse sera by letting immunoglobulins absorb on protein A-Sepharose and by eluting with buffers of decreasing pH. Most donor mice were immunized with a conjugate of a hapten (NIP) and chicken gamma globulin 20 days previously. The results indicate that concentrations of I...

journal_title：Molecular immunology

authors： Sarvas HO,Seppälä IJ,Tähtinen T,Péterfy F,Mäkelä O

更新日期：1983-03-01 00:00:00

abstract：:An efficient method is described for the isolation of highly purified, IgG-free and stable guinea-pig serum C1q. The procedure includes the chromatography of EDTA-treated serum (25 mM EDTA) on CM- and DEAE-cellulose followed by gel filtration on ACA 34-Ultrogel whereby ammonium sulfate precipitation was used for conce...

journal_title：Molecular immunology

authors： Hitschold T,Golan MD,Rabs U,Loos M

更新日期：1983-02-01 00:00:00

abstract：:Tunicamycin inhibited the synthesis of glycosylated mu-chains and kappa-chains in the human lymphoblastoid cell line, Daudi. Nonglycosylated IgM could not be detected on the surface of tunicamycin-treated cells by cell surface iodination techniques even under conditions where membrane IgM was re-expressed in control c...

journal_title：Molecular immunology

authors： Kubo RT,Pelanne ML

更新日期：1983-01-01 00:00:00

abstract：:The allergenic cross-reactivity of cytochromes c isolated from Kentucky bluegrass (KBG) and ryegrass (RG) pollens was demonstrated by the finding that both cytochromes elicited PCA reactions of comparable titers in rats sensitized with murine reaginic antiserum to either KBG or RG cytochrome c. However, allergenic dif...

journal_title：Molecular immunology

authors： Ekramoddoullah AK,Kisil FT,Sehon AH

更新日期：1982-12-01 00:00:00

abstract：:Mice were immunized with a purified preparation of human alpha-foetoprotein (AFP) and their spleen cells were hybridized with mouse myeloma cells. Eleven hybridoma cell lines secreting antibody to AFP were obtained. These antibodies were classified into two groups on the basis of different antigenic determinants they ...

journal_title：Molecular immunology

authors： Nomura M,Imai M,Nakamura T,Miyakawa Y,Mayumi M

更新日期：1982-12-01 00:00:00

abstract：:Normal rat IgG-Sepharose binds the same two receptors from solubilized rat basophilic leukemia (RBL) cells as are bound by rat IgE-Sepharose. These receptors have previously been designated R and H, and have apparent mol. wts of 45,000 and 55,000, respectively. Inhibition studies indicate that, although soluble deaggr...

journal_title：Molecular immunology

authors： Kepron MR,Conrad DH,Froese A

更新日期：1982-12-01 00:00:00

abstract：:The antigenic structural features of alpha-lactalbumin have been investigated using a radioimmunoassay, peptide inhibition of the quantitative precipitin reaction, and by immunodiffusion analysis after chemical modification of the molecule. Antigenic activity (in rabbits) was localized to several peptic fragments and ...

journal_title：Molecular immunology

authors： Hopp TP,Woods KR

更新日期：1982-11-01 00:00:00

abstract：:Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeylle...

journal_title：Molecular immunology

authors： Fountain MW,Dees C,Weete JD,Schultz RD

更新日期：1982-11-01 00:00:00

abstract：:Human complement components C6 and C7 have been purified and characterized. Component C6 is a single-chain plasma protein of mol. wt 104,800 containing 3.8% carbohydrate and it has alanine as the amino terminal residue. Component C7 is a single-chain plasma protein of mol. wt 92,400 containing 6.4% carbohydrate and it...

journal_title：Molecular immunology

authors： Discipio RG,Gagnon J

更新日期：1982-11-01 00:00:00

abstract：:the SC5b-8 complex of human complement is a hydrophilic molecule of mol. wt 800,000-850,000 that is seen in the electron microscope as an elongated, straight or curved structure of 50-55 nm total length and 8-9 nm width. Tryptic attack on the fluid-phase complex exposes lipid-binding surfaces on the molecule. The tryp...

journal_title：Molecular immunology

authors： Bhakdi S,Tranum-Jensen J

更新日期：1982-09-01 00:00:00

abstract：:An antigen-coated plate radioimmunoassay was used to detect antibodies against homologous glutaraldehyde-modified albumin (in monomeric or polymeric form) in normal mouse sera. Mouse antibodies reacted also with heterologous glutaraldehyde-treated albumin; for instance human albumin. Immunoelectrophoresis of purified ...

journal_title：Molecular immunology

authors： Onică D,Mărgineanu I,Medeşan C,Călugăru A,Manciulea M

更新日期：1982-08-01 00:00:00

abstract：:The thermal effects associated with the binding reaction of concanavalin A and immunoglobulins have been measured by calorimetry. IgG solutions do not generate heat on mixing with concanavalin A, confirming the low reactivity of IgG for the lectin molecule. IgM solutions, on the other hand, show a substantial enthalpi...

journal_title：Molecular immunology

authors： Dani M,Manca F,Rialdi G

更新日期：1982-07-01 00:00:00

abstract：:Rabbit antisera specific for horseradish peroxidase inhibit the catalytic activity of the enzyme. All antibodies prepared against the holoenzyme react with the peroxidase apoenzyme. However, only a minority (30-45%) of the total antiperoxidase pool cross react with reduced and alkylated apoenzymes. The antibodies inhi...

journal_title：Molecular immunology

authors： Conroy JM,Salter RD

更新日期：1982-05-01 00:00:00

abstract：:To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with...

journal_title：Molecular immunology

authors： Pisetsky DS,Caster SA

更新日期：1982-05-01 00:00:00

abstract：:The effect of fragment B of staphylococcal protein A on the binding of rabbit IgG to human granulocytes has been examined. When rabbit IgG sensitized sheep erythrocytes (EA) were preincubated with increasing amounts of fragment B, a dose dependent inhibition of rosette formation between the human granulocytes and the ...

journal_title：Molecular immunology

authors： Barnett Foster DE,Sjoquist J,Painter RH

更新日期：1982-03-01 00:00:00

abstract：:Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...

journal_title：Molecular immunology

authors： Bonewald L,Wang AC,Terry E,Zwerner R,Balch CM,Ades EW

更新日期：1982-03-01 00:00:00

abstract：:We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...

journal_title：Molecular immunology

authors： Nishikawa A,Shinoda T,Fukazawa Y

更新日期：1982-03-01 00:00:00

abstract：:A 30,000 mol. wt component (beta) is associated in a 1:1 ratio with the 50,000 mol. wt glycoprotein (alpha) which binds immunoglobulin E (IgE) on mast cells and related tumor cells. We show that alpha and beta are associated in membrane preparations. This is consistent with previous results which showed labeling of be...

journal_title：Molecular immunology

authors： Holowka D,Metzger H

更新日期：1982-02-01 00:00:00

abstract：:The concomitant effects of trans-chromosomal allotype suppression of both an a VH and an n Cmu locus allotype in multiheterozygous rabbits were investigated. For example of the expression of the a2 VH and n81 Cmu allotypes were suppressed in a multiheterozygous rabbit having the a1 chi-y-n81de12,15f73g74/a2 chi 32y33n...

journal_title：Molecular immunology

authors： Horng WJ,Roux KH,Gilman-Sachs A,Dray S

更新日期：1982-01-01 00:00:00